Coding

Part:BBa_K5157012:Design

Designed by: Yueheng Niu   Group: iGEM24_Jiangnan-China   (2024-09-26)


G4S linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A flexible linker, consisting of 4 glycine and 1 serine in a single unit repeated three times, is used to link two structural domains of a fusion protein [1].

Source

The gene of G4S is synthetic, codon optimized and expressed in chassis cell Escherichia coli BL21 (DE3).

References

[1] Chen X, Zaro J L, Shen W C. Fusion protein linkers: property, design and functionality [J]. Advanced Drug Delivery Reviews, 2013, 65(10): 1357-1369.